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Faculty of Biology, Chemistry & Earth Sciences

Research Group Luminescence

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Dose Determination

For the determination of the equivalence dose by means of luminescence different techniques have been developed in recent years. Two fundamentally different basic concepts can be distinguished - the additive and the regenerative method.

By contrast, only one aliquot principally suffices to determine the equivalent dose by regenerative-dose methods (commonly the SAR protocol = single-aliquot regeneration). First, the natural luminescence from this aliquot is measured, before the luminescence signal induced by sequentially administered and variable laboratory doses is registered. The height of the regenerative doses is chosen such that they bracket the naturally accumulated dose. The resulting data (luminescence vs. dose) are fitted and the equivalent dose obtained by projecting the natural signal onto this dose response curve.

By contrast, only one aliquot principally suffices to determine the equivalent dose by regenerative-dose methods (commonly the SAR protocol = single-aliquot regeneration). First, the natural luminescence from this aliquot is measured, before the luminescence signal induced by sequentially administered and variable laboratory doses is registered. The height of the regenerative doses is chosen such that they bracket the naturally accumulated dose. The resulting data (luminescence vs. dose) are fitted and the equivalent dose obtained by projecting the natural signal onto this dose response curve.



To correct for sensitivity changes (change of luminescence output per unit dose) in the course of repeated measurements (see SAR protocol), correction cycles are inserted between each two subsequent regeneration cycles. These correction cycles provide signals for normalisation derived from the application of a constant test dose. The normalised signal which is plotted against regenerative dose is calculated as follows:


Normalised signal = Regeneration signal / Test dose signal


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